Homogeneous Enzymatic Colorimetric Assay for Total Cysteine

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Homogeneous enzymatic colorimetric assay for total cysteine.

Patients with vascular disease have significantly higher concentrations of plasma total cysteine (tCYS) than do healthy individuals (1–7). The present method is a new, rapid, and sensitive enzymatic colorimetric assay for tCYS in plasma samples and is homogeneous in that it avoids separation methods. The tCYS assay uses only the recombinant enzymes methionine , -lyase (rMETase) and Sadenosylhom...

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References 1. Linder MW, Prough RA, Valdes R. Pharmacogenetics: a laboratory tool for optimizing therapeutic efficacy. Clin Chem 1997;43:254–66. 2. Raimundo S, Fischer J, Eichelbaum M, Griese EU, Schwab M, Zanger UM. Elucidation of the genetic basis of the common ‘intermediate metabolizer’ phenotype for drug oxidation by CYP2D6. Pharmacogenetics 2000;10:577– 81. 3. Sachse C, Brockmoller J, Baue...

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ژورنال

عنوان ژورنال: Clinical Chemistry

سال: 2004

ISSN: 0009-9147,1530-8561

DOI: 10.1373/clinchem.2004.032920